DNA Damage

Damage caused by quinolones to DNA. It is suggested that mitochondrial DNA (mtDNA) is the most affected, and this could possibly be a mechanism to the system-wide damages caused by quinolones.

Inhibition of Replication of Chrondrocytes

Posted Fri, 02/06/2009 - 15:36 by FQHelp

Quinolone usage in children has been limited following the observation of arthrotoxicity in juvenile animals... The arthropathy evolves within days to weeks of drug administration... it is hypothesized that the effect of quinolones in some animal species may reflect inhibition of mitochondrial DNA replication in immature chondrocytes

— Allan R. Ronald, Don E. Low

Fluoroquinolone Antibiotics, Birkhäuser, 2003, ISBN 3764365919, 9783764365912

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DNA Gyrase in Mitochondria Affected by Quinolones

Posted Tue, 02/03/2009 - 22:01 by FQHelp

Incorporation of labeled deoxynucleoside triphosphates into mtDNA by isolated rat liver mitochondria has been shown previously to reflect DNA replication. We have used this system to seek evidence for a mtDNA gyrase. Coumermycin, novobiocin, nalidixic acid, and oxolinic acid are known to be inhibitors of Escherichia coli gyrase, to inhibit E. coli DNA replication, to abolish colicin E1 replication, and to depress the supercoiling of phage lambda DNA, the last two via inhibition of the DNA gyrase.

— Frank J. Castora, Melvin V. Simpson, Department of Biochemistry, State University of New York,

http://www.jbc.org/cgi/reprint/254/22/11193.pdf

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Quinolone Induced Mitochondrial DNA Damage

Posted Tue, 02/03/2009 - 21:51 by FQHelp

The loss in mtDNA was associated with a delayed loss in mitochondrial function. Here, we report that the 4-quinolone drug ciprofloxacin is cytotoxic to a variety of cultured mammalian cell lines at concentrations that deplete cells of mtDNA. The IC50 values for ciprofloxacin varied from 40 to 80 micrograms/ml depending on the cell line tested. Cytotoxicity required continuous exposure of cells to drug for 2-4 days, which corresponded to approximately three or four cell doublings. Shorter times of drug exposure did not cause significant cytotoxicity.

— JW Lawrence, DC Claire, V Weissig, TC Rowe, Department of Pharmacology and Therapeutics, University of Florida College of Medicine

http://molpharm.aspetjournals.org/cgi/content/abstract/50/5/1178

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Personal DNA Theory

Posted Tue, 02/03/2009 - 21:05 by FQHelp

The labeling says the reactions are not dose dependent. Any drug that interferes with DNA is dangerous. What if it also interferes with normal cells not only bacterial cells? Our science is not advanced enough to be sure it doesn't. What else could explain the multi systemic ADRs many people have? Collagen disruption is admitted to by the drug maker. Collagen is necessary for healthy tissue in your whole body. I hope doctors think twice, thrice, a hundred times, before ruining someone else's life with quinolones. Anyone who thinks doctor knows best has some growing up to do.

— "Quinned out", Whittier, CA

http://www.topix.com/forum/us/fda/T4C12BQ9L7CVGMQ5I/p4

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Reproductive damage from quinolones

Posted Wed, 01/21/2009 - 13:30 by FQHelp

A significant decrease in both epididymal sperm count and sperm motility besides abnormal spermatozoa rate were observed in enrofloxacin group compared to controls... These results suggested that a fixed 150 mg/kg dose of enrofloxacin would lead disruption of spermatogenesis in the testes causing deterioration of motility and content of sperms as well as morphological abnormalities.

— Aral F, Karaçal F, Baba F

http://www.ncbi.nlm.nih.gov/pubmed/17561208

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